Who Is Leading the Replication Fork, Pol ε or Pol δ?

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Who Is Leading the Replication Fork, Pol ε or Pol δ?

Peter M.J. Burgers,1 Dmitry Gordenin,2 and Thomas A. Kunkel2,* 1Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA 2Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA *Correspondence: [email protected] http://dx.doi.org/10...

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Quality control mechanisms exclude incorrect polymerases from the eukaryotic replication fork.

The eukaryotic genome is primarily replicated by two DNA polymerases, Pol ε and Pol δ, that function on the leading and lagging strands, respectively. Previous studies have established recruitment mechanisms whereby Cdc45-Mcm2-7-GINS (CMG) helicase binds Pol ε and tethers it to the leading strand, and PCNA (proliferating cell nuclear antigen) binds tightly to Pol δ and recruits it to the laggin...

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ژورنال

عنوان ژورنال: Molecular Cell

سال: 2016

ISSN: 1097-2765

DOI: 10.1016/j.molcel.2016.01.017